PoplarV1, Main, Exploration, bibRecord, 001B93

Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation.

Identifieur interne : 001B93 ( Main/Exploration ); précédent : 001B92; suivant : 001B94

Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation.

Auteurs : Xianfeng Tang [République populaire de Chine] ; Yamei Zhuang [République populaire de Chine] ; Guang Qi [République populaire de Chine] ; Dian Wang [République populaire de Chine] ; Huanhuan Liu [République populaire de Chine] ; Kairong Wang [République populaire de Chine] ; Guohua Chai [République populaire de Chine] ; Gongke Zhou [République populaire de Chine]

Source :

Scientific reports [ 2045-2322 ] ; 2015.

RBID : pubmed:26179205

Descripteurs français

KwdFr :
- Bois (MeSH), Données de séquences moléculaires (MeSH), Gènes de plante (MeSH), Paroi cellulaire (métabolisme), Phylogenèse (MeSH), Populus (classification), Populus (génétique), Populus (physiologie), Protéines végétales (composition chimique), Protéines végétales (métabolisme), Protéines végétales (physiologie), Similitude de séquences d'acides aminés (MeSH), Structures de plante (métabolisme), Séquence d'acides aminés (MeSH).
MESH :
- composition chimique : Populus, Protéines végétales.
- génétique : Populus.
- métabolisme : Paroi cellulaire, Protéines végétales, Structures de plante.
- physiologie : Populus, Protéines végétales.
- Bois, Données de séquences moléculaires, Gènes de plante, Phylogenèse, Similitude de séquences d'acides aminés, Séquence d'acides aminés.

English descriptors

KwdEn :
- Amino Acid Sequence (MeSH), Cell Wall (metabolism), Genes, Plant (MeSH), Molecular Sequence Data (MeSH), Phylogeny (MeSH), Plant Proteins (chemistry), Plant Proteins (metabolism), Plant Proteins (physiology), Plant Structures (metabolism), Populus (classification), Populus (genetics), Populus (physiology), Sequence Homology, Amino Acid (MeSH), Wood (MeSH).
MESH :
- chemical , chemistry : Plant Proteins.
- classification : Populus.
- genetics : Populus.
- metabolism : Cell Wall, Plant Proteins, Plant Structures.
- chemical , physiology : Plant Proteins, Populus.
- Amino Acid Sequence, Genes, Plant, Molecular Sequence Data, Phylogeny, Sequence Homology, Amino Acid, Wood.

Abstract

Wood is formed by the successive addition of secondary xylem, which consists of cells with a conspicuously thickened secondary wall composed mainly of cellulose, xylan and lignin. Currently, few transcription factors involved in the direct regulation of secondary wall biosynthesis have been characterized in tree species. Here, we show that PdMYB221, a poplar ortholog of the Arabidopsis R2R3-MYB transcription factor AtMYB4, directly regulates secondary wall biosynthesis during wood formation. PdMYB221 is predominantly expressed in cells of developing wood, and the protein it encodes localizes to the nucleus and acts as a transcriptional repressor. Ectopic expression of PdMYB221 resulted in reduced cell wall thicknesses of fibers and vessels in Arabidopsis inflorescence stems. The amounts of cellulose, xylose, and lignin were decreased and the expression of key genes synthesizing the three components was suppressed in PdMYB221 overexpression plants. Transcriptional activation assays showed that PdMYB221 repressed the promoters of poplar PdCESA7/8, PdGT47C, PdCOMT2 and PdCCR1. Electrophoretic mobility shift assays revealed that PdMYB221 bound directly to the PdCESA8, PdGT47C, and PdCOMT2 promoters. Together, our results suggest that PdMYB221 may be involved in the negative regulation of secondary wall formation through the direct and indirect suppression of the gene expression of secondary wall biosynthesis.

DOI: 10.1038/srep12240
PubMed: 26179205
PubMed Central: PMC4503951

Affiliations:

République populaire de Chine

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<term>Cell Wall (metabolism)</term>
<term>Genes, Plant (MeSH)</term>
<term>Molecular Sequence Data (MeSH)</term>
<term>Phylogeny (MeSH)</term>
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<term>Plant Proteins (physiology)</term>
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<term>Paroi cellulaire (métabolisme)</term>
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<term>Protéines végétales (physiologie)</term>
<term>Similitude de séquences d'acides aminés (MeSH)</term>
<term>Structures de plante (métabolisme)</term>
<term>Séquence d'acides aminés (MeSH)</term>
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<term>Similitude de séquences d'acides aminés</term>
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<front><div type="abstract" xml:lang="en">Wood is formed by the successive addition of secondary xylem, which consists of cells with a conspicuously thickened secondary wall composed mainly of cellulose, xylan and lignin. Currently, few transcription factors involved in the direct regulation of secondary wall biosynthesis have been characterized in tree species. Here, we show that PdMYB221, a poplar ortholog of the Arabidopsis R2R3-MYB transcription factor AtMYB4, directly regulates secondary wall biosynthesis during wood formation. PdMYB221 is predominantly expressed in cells of developing wood, and the protein it encodes localizes to the nucleus and acts as a transcriptional repressor. Ectopic expression of PdMYB221 resulted in reduced cell wall thicknesses of fibers and vessels in Arabidopsis inflorescence stems. The amounts of cellulose, xylose, and lignin were decreased and the expression of key genes synthesizing the three components was suppressed in PdMYB221 overexpression plants. Transcriptional activation assays showed that PdMYB221 repressed the promoters of poplar PdCESA7/8, PdGT47C, PdCOMT2 and PdCCR1. Electrophoretic mobility shift assays revealed that PdMYB221 bound directly to the PdCESA8, PdGT47C, and PdCOMT2 promoters. Together, our results suggest that PdMYB221 may be involved in the negative regulation of secondary wall formation through the direct and indirect suppression of the gene expression of secondary wall biosynthesis. </div>
</front>
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<ArticleTitle>Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation.</ArticleTitle>
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<Abstract><AbstractText>Wood is formed by the successive addition of secondary xylem, which consists of cells with a conspicuously thickened secondary wall composed mainly of cellulose, xylan and lignin. Currently, few transcription factors involved in the direct regulation of secondary wall biosynthesis have been characterized in tree species. Here, we show that PdMYB221, a poplar ortholog of the Arabidopsis R2R3-MYB transcription factor AtMYB4, directly regulates secondary wall biosynthesis during wood formation. PdMYB221 is predominantly expressed in cells of developing wood, and the protein it encodes localizes to the nucleus and acts as a transcriptional repressor. Ectopic expression of PdMYB221 resulted in reduced cell wall thicknesses of fibers and vessels in Arabidopsis inflorescence stems. The amounts of cellulose, xylose, and lignin were decreased and the expression of key genes synthesizing the three components was suppressed in PdMYB221 overexpression plants. Transcriptional activation assays showed that PdMYB221 repressed the promoters of poplar PdCESA7/8, PdGT47C, PdCOMT2 and PdCCR1. Electrophoretic mobility shift assays revealed that PdMYB221 bound directly to the PdCESA8, PdGT47C, and PdCOMT2 promoters. Together, our results suggest that PdMYB221 may be involved in the negative regulation of secondary wall formation through the direct and indirect suppression of the gene expression of secondary wall biosynthesis. </AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tang</LastName>
<ForeName>Xianfeng</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>Key Laboratory of Biofuels, Chinese Academy of Sciences, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhuang</LastName>
<ForeName>Yamei</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>1] Key Laboratory of Biofuels, Chinese Academy of Sciences, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China [2] Qingdao Engineering Research Center for Rural Environment, College of Resources and Environment, Qingdao Agricultural University, Qingdao, 266109, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Qi</LastName>
<ForeName>Guang</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Key Laboratory of Biofuels, Chinese Academy of Sciences, Shandong Provincial Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, 266101, China.</Affiliation>
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<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Dian</ForeName>
<Initials>D</Initials>
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</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Liu</LastName>
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<affiliations><list><country><li>République populaire de Chine</li>
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Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation.

Poplar PdMYB221 is involved in the direct and indirect regulation of secondary wall biosynthesis during wood formation.

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